5H5Q

Crystal structure of human GPX4 in complex with GXpep-1

5H5Q の概要

• エントリーDOI: 10.2210/pdb5h5q/pdb
• 関連するPDBエントリー: 5H5R 5H5S
• 分子名称: Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GXpep-1, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: phospholipid hydroperoxide glutathione peroxidase 4, selenocysteine, oxidoreductase, oxidoreductase-oxidoreductase inhibitor complex, oxidoreductase/oxidoreductase inhibitor
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm: P36969
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21336.34

構造登録者

Sogabe, S.,Kadotani, A.,Lane, W.,Snell, G. (登録日: 2016-11-09, 公開日: 2016-12-07, 最終更新日: 2023-11-15)

主引用文献

Sakamoto, K.,Sogabe, S.,Kamada, Y.,Matsumoto, S.I.,Kadotani, A.,Sakamoto, J.I.,Tani, A.
Discovery of GPX4 inhibitory peptides from random peptide T7 phage display and subsequent structural analysis
Biochem. Biophys. Res. Commun., 482:195-201, 2017

PubMed Abstract: The phospholipid hydroperoxidase glutathione peroxidase (GPX4) is an enzyme that reduces lipid hydroperoxides in lipid membranes. Recently, GPX4 has been investigated as a target molecule that induces iron-dependent cell death (ferroptosis) selectively in cancer cells that express mutant Ras. GPX4 inhibitors have the potential to become novel anti-cancer drugs. However, there are no druggable pockets for conventional small molecules on the molecular surface of GPX4. To generate GPX4 inhibitors, we examined the use of peptides as an alternative to small molecules. By screening peptide libraries displayed on T7 phages, and analyzing the X-ray crystal structures of the peptides, we successfully identified one peptide that binds to near Sec73 of catalytic site and two peptides that bind to another site on GPX4. To our knowledge, this is the first study reporting GPX4 inhibitory peptides and their structural information.

PubMed: 27836545
DOI: 10.1016/j.bbrc.2016.11.035

実験手法

X-RAY DIFFRACTION (1.1 Å)