5H2P
A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 657 us after photoexcitation
Summary for 5H2P
| Entry DOI | 10.2210/pdb5h2p/pdb |
| Related | 5B6V 5B6W 5B6X 5B6Y 5B6Z 5H2H 5H2I 5H2J 5H2K 5H2L 5H2M 5H2N 5H2O |
| Descriptor | Bacteriorhodopsin, nonane, TETRADECANE, ... (12 entities in total) |
| Functional Keywords | bacteriorhodopsin, xfel, time-resolved serial femtosecond crystallography, sacla, proton transport |
| Biological source | Halobacterium salinarum NRC-1 (Halobacterium sp. NRC-1) |
| Cellular location | Cell membrane ; Multi-pass membrane protein : P02945 |
| Total number of polymer chains | 1 |
| Total formula weight | 30891.94 |
| Authors | Royant, A.,Nango, E.,Nakane, T.,Tanaka, T.,Arima, T.,Neutze, R.,Iwata, S. (deposition date: 2016-10-15, release date: 2016-12-21, Last modification date: 2023-11-08) |
| Primary citation | Nango, E.,Royant, A.,Kubo, M.,Nakane, T.,Wickstrand, C.,Kimura, T.,Tanaka, T.,Tono, K.,Song, C.,Tanaka, R.,Arima, T.,Yamashita, A.,Kobayashi, J.,Hosaka, T.,Mizohata, E.,Nogly, P.,Sugahara, M.,Nam, D.,Nomura, T.,Shimamura, T.,Im, D.,Fujiwara, T.,Yamanaka, Y.,Jeon, B.,Nishizawa, T.,Oda, K.,Fukuda, M.,Andersson, R.,Bath, P.,Dods, R.,Davidsson, J.,Matsuoka, S.,Kawatake, S.,Murata, M.,Nureki, O.,Owada, S.,Kameshima, T.,Hatsui, T.,Joti, Y.,Schertler, G.,Yabashi, M.,Bondar, A.N.,Standfuss, J.,Neutze, R.,Iwata, S. A three-dimensional movie of structural changes in bacteriorhodopsin Science, 354:1552-1557, 2016 Cited by PubMed Abstract: Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient. PubMed: 28008064DOI: 10.1126/science.aah3497 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.1 Å) |
Structure validation
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