5H2P

A three dimensional movie of structural changes in bacteriorhodopsin: structure obtained 657 us after photoexcitation

5H2P の概要

• エントリーDOI: 10.2210/pdb5h2p/pdb
• 関連するPDBエントリー: 5B6V 5B6W 5B6X 5B6Y 5B6Z 5H2H 5H2I 5H2J 5H2K 5H2L 5H2M 5H2N 5H2O
• 分子名称: Bacteriorhodopsin, nonane, TETRADECANE, ... (12 entities in total)
• 機能のキーワード: bacteriorhodopsin, xfel, time-resolved serial femtosecond crystallography, sacla, proton transport
• 由来する生物種: Halobacterium salinarum NRC-1 (Halobacterium sp. NRC-1)
• 細胞内の位置: Cell membrane ; Multi-pass membrane protein : P02945
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30891.94

構造登録者

Royant, A.,Nango, E.,Nakane, T.,Tanaka, T.,Arima, T.,Neutze, R.,Iwata, S. (登録日: 2016-10-15, 公開日: 2016-12-21, 最終更新日: 2023-11-08)

主引用文献

Nango, E.,Royant, A.,Kubo, M.,Nakane, T.,Wickstrand, C.,Kimura, T.,Tanaka, T.,Tono, K.,Song, C.,Tanaka, R.,Arima, T.,Yamashita, A.,Kobayashi, J.,Hosaka, T.,Mizohata, E.,Nogly, P.,Sugahara, M.,Nam, D.,Nomura, T.,Shimamura, T.,Im, D.,Fujiwara, T.,Yamanaka, Y.,Jeon, B.,Nishizawa, T.,Oda, K.,Fukuda, M.,Andersson, R.,Bath, P.,Dods, R.,Davidsson, J.,Matsuoka, S.,Kawatake, S.,Murata, M.,Nureki, O.,Owada, S.,Kameshima, T.,Hatsui, T.,Joti, Y.,Schertler, G.,Yabashi, M.,Bondar, A.N.,Standfuss, J.,Neutze, R.,Iwata, S.
A three-dimensional movie of structural changes in bacteriorhodopsin
Science, 354:1552-1557, 2016

PubMed Abstract: Bacteriorhodopsin (bR) is a light-driven proton pump and a model membrane transport protein. We used time-resolved serial femtosecond crystallography at an x-ray free electron laser to visualize conformational changes in bR from nanoseconds to milliseconds following photoactivation. An initially twisted retinal chromophore displaces a conserved tryptophan residue of transmembrane helix F on the cytoplasmic side of the protein while dislodging a key water molecule on the extracellular side. The resulting cascade of structural changes throughout the protein shows how motions are choreographed as bR transports protons uphill against a transmembrane concentration gradient.

PubMed: 28008064
DOI: 10.1126/science.aah3497

実験手法

X-RAY DIFFRACTION (2.1 Å)