5H1C
Human RAD51 post-synaptic complexes
Summary for 5H1C
Entry DOI | 10.2210/pdb5h1c/pdb |
Related | 5H1B |
EMDB information | 9566 9567 9568 |
Descriptor | DNA repair protein RAD51 homolog 1, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), ... (5 entities in total) |
Functional Keywords | dna repair, atpase, homologous recombination, dna binding protein-dna complex, dna binding protein/dna |
Biological source | Homo sapiens (Human) More |
Cellular location | Nucleus : Q06609 |
Total number of polymer chains | 5 |
Total formula weight | 118082.41 |
Authors | |
Primary citation | Xu, J.,Zhao, L.,Xu, Y.,Zhao, W.,Sung, P.,Wang, H.W. Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange Nat. Struct. Mol. Biol., 24:40-46, 2017 Cited by PubMed Abstract: The central step in eukaryotic homologous recombination (HR) is ATP-dependent DNA-strand exchange mediated by the Rad51 recombinase. In this process, Rad51 assembles on single-stranded DNA (ssDNA) and generates a helical filament that is able to search for and invade homologous double-stranded DNA (dsDNA), thus leading to strand separation and formation of new base pairs between the initiating ssDNA and the complementary strand within the duplex. Here, we used cryo-EM to solve the structures of human RAD51 in complex with DNA molecules, in presynaptic and postsynaptic states, at near-atomic resolution. Our structures reveal both conserved and distinct structural features of the human RAD51-DNA complexes compared with their prokaryotic counterpart. Notably, we also captured the structure of an arrested synaptic complex. Our results provide new insight into the molecular mechanisms of the DNA homology search and strand-exchange processes. PubMed: 27941862DOI: 10.1038/nsmb.3336 PDB entries with the same primary citation |
Experimental method | ELECTRON MICROSCOPY (4.5 Å) |
Structure validation
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