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5H1C

Human RAD51 post-synaptic complexes

Summary for 5H1C
Entry DOI10.2210/pdb5h1c/pdb
Related5H1B
EMDB information9566 9567 9568
DescriptorDNA repair protein RAD51 homolog 1, DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*A)-3'), ... (5 entities in total)
Functional Keywordsdna repair, atpase, homologous recombination, dna binding protein-dna complex, dna binding protein/dna
Biological sourceHomo sapiens (Human)
More
Cellular locationNucleus : Q06609
Total number of polymer chains5
Total formula weight118082.41
Authors
Xu, J.,Zhao, L.,Xu, Y.,Zhao, W.,Sung, P.,Wang, H.W. (deposition date: 2016-10-08, release date: 2016-12-21, Last modification date: 2024-10-09)
Primary citationXu, J.,Zhao, L.,Xu, Y.,Zhao, W.,Sung, P.,Wang, H.W.
Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange
Nat. Struct. Mol. Biol., 24:40-46, 2017
Cited by
PubMed Abstract: The central step in eukaryotic homologous recombination (HR) is ATP-dependent DNA-strand exchange mediated by the Rad51 recombinase. In this process, Rad51 assembles on single-stranded DNA (ssDNA) and generates a helical filament that is able to search for and invade homologous double-stranded DNA (dsDNA), thus leading to strand separation and formation of new base pairs between the initiating ssDNA and the complementary strand within the duplex. Here, we used cryo-EM to solve the structures of human RAD51 in complex with DNA molecules, in presynaptic and postsynaptic states, at near-atomic resolution. Our structures reveal both conserved and distinct structural features of the human RAD51-DNA complexes compared with their prokaryotic counterpart. Notably, we also captured the structure of an arrested synaptic complex. Our results provide new insight into the molecular mechanisms of the DNA homology search and strand-exchange processes.
PubMed: 27941862
DOI: 10.1038/nsmb.3336
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (4.5 Å)
Structure validation

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