5GSW

Crystal structure of EV71 3C in complex with N69S 1.8k

Summary for 5GSW

• Entry DOI: 10.2210/pdb5gsw/pdb
• Related: 5GSO
• Descriptor: 3C protein, ~{N}-[(2~{S})-3-(4-fluorophenyl)-1-oxidanylidene-1-[[(2~{S})-1-oxidanylidene-3-[(3~{S})-2-oxidanylidenepiperidin-3-yl]propan-2-yl]amino]propan-2-yl]-5-methyl-1,2-oxazole-3-carboxamide (3 entities in total)
• Functional Keywords: protease, hydrolase
• Biological source: Enterovirus A71
• Total number of polymer chains: 5
• Total formula weight: 103032.82

Authors

Wang, Y. (deposition date: 2016-08-17, release date: 2017-05-31, Last modification date: 2024-03-20)

Primary citation

Wang, Y.,Cao, L.,Zhai, Y.,Yin, Z.,Sun, Y.,Shang, L.
Structure of the Enterovirus 71 3C Protease in Complex with NK-1.8k and Indications for the Development of Antienterovirus Protease Inhibitor
Antimicrob. Agents Chemother., 61:-, 2017

PubMed Abstract: Hand-foot-and-mouth disease (HFMD), caused by enterovirus, is a threat to public health worldwide. To date, enterovirus 71 (EV71) has been one of the major causative agents of HFMD in the Pacific-Asia region, and outbreaks with EV71 cause millions of infections. However, no drug is currently available for clinical therapeutics. In our previous works, we developed a set of protease inhibitors (PIs) targeting the EV71 3C protease (3C). Among these are NK-1.8k and NK-1.9k, which have various active groups and high potencies and selectivities. In the study described here, we determined the structures of the PI NK-1.8k in complex with wild-type (WT) and drug-resistant EV71 3C Comparison of these structures with the structure of unliganded EV71 3C and its complex with AG7088 indicated that the mutation of N69 to a serine residue destabilized the S2 pocket. Thus, the mutation influenced the cleavage activity of EV71 3C and the inhibitory activity of NK-1.8k in an protease assay and highlighted that site 69 is an additional key site for PI design. More information for the optimization of the P1' to P4 groups of PIs was also obtained from these structures. Together with the results of our previous works, these in-depth results elucidate the inhibitory mechanism of PIs and shed light to develop PIs for the clinical treatment of infections caused by EV71 and other enteroviruses.

PubMed: 28461310
DOI: 10.1128/AAC.00298-17

Experimental method

X-RAY DIFFRACTION (3.19 Å)