5GQR

Crystal structure of PXY-CLE41-SERK2

Summary for 5GQR

• Entry DOI: 10.2210/pdb5gqr/pdb
• Descriptor: Leucine-rich repeat receptor-like protein kinase TDR, TDIF, Somatic embryogenesis receptor kinase 2, ... (4 entities in total)
• Functional Keywords: meristem cell proliferation, serk, cle peptides, leucine rich repeat, transferase
• Biological source: Arabidopsis thaliana (Mouse-ear cress); More
• Total number of polymer chains: 3
• Total formula weight: 88194.26

Authors

Chai, J.J.,Zhang, H.Q. (deposition date: 2016-08-08, release date: 2016-12-14, Last modification date: 2023-11-08)

Primary citation

Zhang, H.Q.,Lin, X.Y.,Han, Z.F.,Wang, J.,Qu, L.J.,Chai, J.J.
SERK Family Receptor-like Kinases Function as Co-receptors with PXY for Plant Vascular Development
Mol Plant, 9:1406-1414, 2016

PubMed Abstract: In Arabidopsis, the CLAVATA3/EMBRYO SURROUNDING REGION-RELATED (CLE) peptides play important roles in regulating proliferation and differentiation of plant-specific stem cells. Although receptors of CLEs are reported to be leucine-rich repeat receptor kinases, the mechanisms underlying CLE-induced receptor activation remain largely unknown. Here we show that SOMATIC EMBRYOGENESIS RECEPTOR KINASEs (SERKs) serve as co-receptors in CLE41/TDIF-PXY signaling to regulate plant vascular development. TDIF induces interaction of its receptor PXY with SERKs in vitro and in vivo. Furthermore, the serk1-1 serk2-1 bak1-5 mutant plants are less sensitive to TDIF, phenocopying the pxy mutant with a compromised promotion of procambial cell proliferation. Crystal structure of the PXY-TDIF-SERK2 complex reveals that the last amino acid of TDIF conserved among CLEs and other evolutionary-related peptides is important for the interaction between SERK2 and PXY. Taken together, our current study identifies SERKs as signaling components of the TDIF-PXY pathway and suggests a conserved activation mechanism of CLE receptors.

PubMed: 27449136
DOI: 10.1016/j.molp.2016.07.004

Experimental method

X-RAY DIFFRACTION (3.5 Å)