5GQD

Crystal structure of covalent glycosyl-enzyme intermediate of xylanase mutant (T82A, N127S, and E128H) from Streptomyces olivaceoviridis E-86

Summary for 5GQD

• Entry DOI: 10.2210/pdb5gqd/pdb
• Related: 5GQE
• Descriptor: Beta-xylanase, beta-D-xylopyranose-(1-4)-alpha-D-xylopyranose, GLYCEROL, ... (4 entities in total)
• Functional Keywords: xylanase, mutant, pnp-xylobioside, covalent glycosyl-enzyme intermediate, hydrolase
• Biological source: Streptomyces olivaceoviridis
• Total number of polymer chains: 2
• Total formula weight: 94787.66

Authors

Suzuki, R.,Fujimoto, Z.,Kaneko, S.,Kuno, A. (deposition date: 2016-08-07, release date: 2017-08-09, Last modification date: 2023-11-08)

• Primary citation: Suzuki, R.,Fujimoto, Z.,Kaneko, S.,Hasegawa, T.,Kuno, A.; Azidolysis by the Formation of Stable Ser-His Catalytic Dyad in a Glycoside Hydrolase Family 10 Xylanase Mutant; J.Appl.Glyosci., 65:-, 2019; DOI: 10.5458/jag.jag.JAG-2017_011

Experimental method

X-RAY DIFFRACTION (1.8 Å)