5GQD
Crystal structure of covalent glycosyl-enzyme intermediate of xylanase mutant (T82A, N127S, and E128H) from Streptomyces olivaceoviridis E-86
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-xylanase | polymer | 436 | 46743.2 | 2 | UniProt (Q7SI98) Pfam (PF00331) Pfam (PF00652) | Streptomyces olivaceoviridis | |
| 2 | C, D (C, D) | beta-D-xylopyranose-(1-4)-alpha-D-xylopyranose | branched | 282.2 | 2 | In PDB GlyTouCan (G73335RX) | |||
| 3 | E, F, G, H, I... (A, B) | GLYCEROL | non-polymer | 92.1 | 8 | Chemie (GOL) | |||
| 4 | M, N (A, B) | water | water | 18.0 | 1001 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 427 (UniProt: Q7SI98)
| PDB | External Database | Details |
|---|---|---|
| Ala 82 | Thr 82 | engineered mutation |
| Ser 127 | Asn 127 | engineered mutation |
| His 128 | Glu 128 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 93486.4 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 564.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 736.8 | |
| All* | Total formula weight | 94787.7 |
