5GAN

The overall structure of the yeast spliceosomal U4/U6.U5 tri-snRNP at 3.7 Angstrom

5GAN の概要

• エントリーDOI: 10.2210/pdb5gan/pdb
• EMDBエントリー: 8012
• 分子名称: U4 snRNA, Unknown protein, Small nuclear ribonucleoprotein-associated protein B, ... (29 entities in total)
• 機能のキーワード: pre-mrna splicing, snrnp, gtpase, u5 snrna, prp8, spliceosome, u4/u6 snrnp, brr2, snu114, transcription
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1404066.13

構造登録者

Nguyen, T.H.D.,Galej, W.P.,Bai, X.C.,Oubridge, C.,Scheres, S.H.W.,Newman, A.J.,Nagai, K. (登録日: 2015-12-15, 公開日: 2016-01-27, 最終更新日: 2024-05-15)

主引用文献

Nguyen, T.H.,Galej, W.P.,Bai, X.C.,Oubridge, C.,Newman, A.J.,Scheres, S.H.,Nagai, K.
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 angstrom resolution.
Nature, 530:298-302, 2016

PubMed Abstract: U4/U6.U5 tri-snRNP represents a substantial part of the spliceosome before activation. A cryo-electron microscopy structure of Saccharomyces cerevisiae U4/U6.U5 tri-snRNP at 3.7 Å resolution led to an essentially complete atomic model comprising 30 proteins plus U4/U6 and U5 small nuclear RNAs (snRNAs). The structure reveals striking interweaving interactions of the protein and RNA components, including extended polypeptides penetrating into subunit interfaces. The invariant ACAGAGA sequence of U6 snRNA, which base-pairs with the 5'-splice site during catalytic activation, forms a hairpin stabilized by Dib1 and Prp8 while the adjacent nucleotides interact with the exon binding loop 1 of U5 snRNA. Snu114 harbours GTP, but its putative catalytic histidine is held away from the γ-phosphate by hydrogen bonding to a tyrosine in the amino-terminal domain of Prp8. Mutation of this histidine to alanine has no detectable effect on yeast growth. The structure provides important new insights into the spliceosome activation process leading to the formation of the catalytic centre.

PubMed: 26829225
DOI: 10.1038/nature16940

実験手法

ELECTRON MICROSCOPY (3.7 Å)