5FW2
Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA
Summary for 5FW2
Entry DOI | 10.2210/pdb5fw2/pdb |
Related | 5FW1 5FW3 |
Descriptor | SGRNA, CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, TARGET DNA STRAND, ... (7 entities in total) |
Functional Keywords | hydrolase-dna-rna complex, crispr, cas9, endonuclease, pam, genome editing, rnp, protein-rna complex, hydrolase/dna/rna |
Biological source | STREPTOCOCCUS PYOGENES More |
Total number of polymer chains | 4 |
Total formula weight | 198422.66 |
Authors | Anders, C.,Bargsten, K.,Jinek, M. (deposition date: 2016-02-11, release date: 2016-06-15, Last modification date: 2024-01-10) |
Primary citation | Anders, C.,Bargsten, K.,Jinek, M. Structural Plasticity of Pam Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9. Mol.Cell, 61:895-, 2016 Cited by PubMed: 26990992DOI: 10.1016/J.MOLCEL.2016.02.020 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.676 Å) |
Structure validation
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