5FW2
Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-22 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.740, 68.240, 188.220 |
| Unit cell angles | 90.00, 110.95, 90.00 |
Refinement procedure
| Resolution | 48.251 - 2.676 |
| R-factor | 0.2064 |
| Rwork | 0.205 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4un3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.658 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 2.750 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.120 | 0.770 |
| Number of reflections | 59682 | |
| <I/σ(I)> | 13.2 | 2.2 |
| Completeness [%] | 99.3 | 91.7 |
| Redundancy | 7 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS PH 8.5 0.3M KSCN 15% (W/V) PEG 3400 |
