5FVA

Toscana Virus Nucleocapsid Protein

Summary for 5FVA

• Entry DOI: 10.2210/pdb5fva/pdb
• Descriptor: NUCLEOPROTEIN, SODIUM ION (3 entities in total)
• Functional Keywords: viral protein, toscana virus, nucleocapside, nucleoprotein, phlebovirus, rna virus, bunyaviridae
• Biological source: TOSCANA VIRUS
• Cellular location: Virion: P21701
• Total number of polymer chains: 6
• Total formula weight: 166110.75

Authors

Baklouti, A.,Sevajol, M.,Goulet, A.,Lichiere, J.,Ferron, F.,Canard, B.,Charrel, R.N.,Coutard, B.,Papageorgiou, N. (deposition date: 2016-02-03, release date: 2016-03-09, Last modification date: 2024-01-10)

Primary citation

Baklouti, A.,Goulet, A.,Lichiere, J.,Canard, B.,Charrel, R.N.,Ferron, F.,Coutard, B.,Papageorgiou, N.
Toscana virus nucleoprotein oligomer organization observed in solution.
Acta Crystallogr D Struct Biol, 73:650-659, 2017

PubMed Abstract: Toscana virus (TOSV) is an arthropod-borne virus belonging to the Phlebovirus genus within the Bunyaviridae family. As in other bunyaviruses, the genome of TOSV is made up of three RNA segments. They are encapsidated by the nucleoprotein (N), which also plays an essential role in virus replication. To date, crystallographic structures of phlebovirus N have systematically revealed closed-ring organizations which do not fully match the filamentous organization of the ribonucleoprotein (RNP) complex observed by electron microscopy. In order to further bridge the gap between crystallographic data on N and observations of the RNP by electron microscopy, the structural organization of recombinant TOSV N was investigated by an integrative approach combining X-ray diffraction crystallography, transmission electron microscopy, small-angle X-ray scattering, size-exclusion chromatography and multi-angle laser light scattering. It was found that in solution TOSV N forms open oligomers consistent with the encapsidation mechanism of phlebovirus RNA.

PubMed: 28777080
DOI: 10.1107/S2059798317008774

Experimental method

X-RAY DIFFRACTION (3.7 Å)