5FVA
Toscana Virus Nucleocapsid Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2015-01-30 |
| Detector | EIGER 4M |
| Spacegroup name | P 6 |
| Unit cell lengths | 174.615, 174.615, 89.824 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.222 - 3.700 |
| R-factor | 0.2341 |
| Rwork | 0.228 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB MODEL 4CSG |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.477 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.220 | 3.730 |
| High resolution limit [Å] | 3.700 | 3.600 |
| Rmerge | 0.210 | 1.230 |
| Number of reflections | 18291 | |
| <I/σ(I)> | 10.48 | 1.99 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.5 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
