5FQ5
Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing
Summary for 5FQ5
| Entry DOI | 10.2210/pdb5fq5/pdb |
| Descriptor | SGRNA, CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1, TARGET DNA STRAND PROXIMAL FRAGMENT, ... (8 entities in total) |
| Functional Keywords | hydrolase-dna complex, crispr, cas9, genome editing, protein-rna complex, hydrolase/dna |
| Biological source | STREPTOCOCCUS PYOGENES More |
| Total number of polymer chains | 5 |
| Total formula weight | 198470.95 |
| Authors | Olieric, V.,Weinert, T.,Finke, A.,Anders, C.,Jinek, M.,Wang, M. (deposition date: 2015-12-07, release date: 2016-03-23, Last modification date: 2019-10-23) |
| Primary citation | Olieric, V.,Weinert, T.,Finke, A.D.,Anders, C.,Li, D.,Olieric, N.,Borca, C.N.,Steinmetz, M.O.,Caffrey, M.,Jinek, M.,Wang, M. Data-Collection Strategy for Challenging Native Sad Phasing. Acta Crystallogr.,Sect.D, 72:421-, 2016 Cited by PubMed: 26960129DOI: 10.1107/S2059798315024110 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.136 Å) |
Structure validation
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