5FG3
Crystal structure of GDP-bound aIF5B from Aeropyrum pernix
Summary for 5FG3
| Entry DOI | 10.2210/pdb5fg3/pdb |
| Descriptor | Probable translation initiation factor IF-2, GUANOSINE-5'-DIPHOSPHATE (3 entities in total) |
| Functional Keywords | translation initiaton factor, gtpase, ribosomal subunit joining, crenarchaea, translation |
| Biological source | Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) |
| Total number of polymer chains | 1 |
| Total formula weight | 69898.92 |
| Authors | Murakami, R.,Miyoshi, T.,Uchiumi, T.,Ito, K. (deposition date: 2015-12-20, release date: 2016-05-04, Last modification date: 2023-11-08) |
| Primary citation | Murakami, R.,Miyoshi, T.,Uchiumi, T.,Ito, K. Crystal structure of translation initiation factor 5B from the crenarchaeon Aeropyrum pernix. Proteins, 84:712-717, 2016 Cited by PubMed Abstract: Initiation factor 5B (IF5B) is a universally conserved translational GTPase that catalyzes ribosomal subunit joining. In eukaryotes, IF5B directly interacts via a groove in its domain IV with initiation factor 1A (IF1A), another universally conserved initiation factor, to accomplish efficient subunit joining. Here, we have determined the first structure of a crenarchaeal IF5B, which revealed that the archaea-specific region of IF5B (helix α15) binds and occludes the groove of domain IV. Therefore, archaeal IF5B cannot access IF1A in the same manner as eukaryotic IF5B. This fact suggests that different relationships between IF5B and IF1A exist in archaea and eukaryotes. PubMed: 26868175DOI: 10.1002/prot.25009 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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