5FG3
Crystal structure of GDP-bound aIF5B from Aeropyrum pernix
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-11-24 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.853, 97.448, 61.296 |
Unit cell angles | 90.00, 94.77, 90.00 |
Refinement procedure
Resolution | 37.210 - 1.900 |
R-factor | 0.19668 |
Rwork | 0.194 |
R-free | 0.24293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g7s |
RMSD bond length | 0.010 |
RMSD bond angle | 1.446 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.210 | 2.020 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.065 | 0.305 |
Number of reflections | 50764 | |
<I/σ(I)> | 12.3 | 3.7 |
Completeness [%] | 95.4 | 88.1 |
Redundancy | 3.5 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Tris-HCl (pH7.5), 20%(w/v) PEG 3000, 0.2 M calcium acetate |