5F1B

Structural basis of Ebola virus entry: viral glycoprotein bound to its endosomal receptor Niemann-Pick C1

5F1B の概要

• エントリーDOI: 10.2210/pdb5f1b/pdb
• 関連するPDBエントリー: 5F18
• 分子名称: GP1, GP2, Niemann-Pick C1 protein, ... (5 entities in total)
• 機能のキーワード: ebola virus, glycoprotein, npc1-c, viral protein-transport protein complex, viral protein/transport protein
• 由来する生物種: Zaire ebolavirus (ZEBOV); 詳細
• 細胞内の位置: GP2: Virion membrane ; Single- pass type I membrane protein . GP1: Virion membrane ; Peripheral membrane protein . GP2-delta: Secreted : P87666 P87666; Late endosome membrane ; Multi-pass membrane protein : O15118
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 61938.33

構造登録者

Wang, H.,Shi, Y.,Song, J.,Qi, J.,Lu, G.,Yan, J.,Gao, G.F. (登録日: 2015-11-30, 公開日: 2016-01-20, 最終更新日: 2024-10-09)

主引用文献

Wang, H.,Shi, Y.,Song, J.,Qi, J.,Lu, G.,Yan, J.,Gao, G.F.
Ebola Viral Glycoprotein Bound to Its Endosomal Receptor Niemann-Pick C1.
Cell, 164:258-268, 2016

PubMed Abstract: Filoviruses, including Ebola and Marburg, cause fatal hemorrhagic fever in humans and primates. Understanding how these viruses enter host cells could help to develop effective therapeutics. An endosomal protein, Niemann-Pick C1 (NPC1), has been identified as a necessary entry receptor for this process, and priming of the viral glycoprotein (GP) to a fusion-competent state is a prerequisite for NPC1 binding. Here, we have determined the crystal structure of the primed GP (GPcl) of Ebola virus bound to domain C of NPC1 (NPC1-C) at a resolution of 2.3 Å. NPC1-C utilizes two protruding loops to engage a hydrophobic cavity on head of GPcl. Upon enzymatic cleavage and NPC1-C binding, conformational change in the GPcl further affects the state of the internal fusion loop, triggering membrane fusion. Our data therefore provide structural insights into filovirus entry in the late endosome and the molecular basis for design of therapeutic inhibitors of viral entry.

PubMed: 26771495
DOI: 10.1016/j.cell.2015.12.044

実験手法

X-RAY DIFFRACTION (2.3 Å)