5EZC

A de novo designed heptameric coiled coil CC-Hept-I18C-L22H-I25E

Summary for 5EZC

• Entry DOI: 10.2210/pdb5ezc/pdb
• Related: 4pna
• Descriptor: CC-Hept-C-H-E (2 entities in total)
• Functional Keywords: coiled coil, heptamer, de novo protein
• Biological source: synthetic construct
• Total number of polymer chains: 7
• Total formula weight: 23673.66

Authors

Burton, A.J.,Brady, R.L.,Woolfson, D.N. (deposition date: 2015-11-26, release date: 2016-07-06, Last modification date: 2024-11-20)

Primary citation

Burton, A.J.,Thomson, A.R.,Dawson, W.M.,Brady, R.L.,Woolfson, D.N.
Installing hydrolytic activity into a completely de novo protein framework.
Nat.Chem., 8:837-844, 2016

PubMed Abstract: The design of enzyme-like catalysts tests our understanding of sequence-to-structure/function relationships in proteins. Here we install hydrolytic activity predictably into a completely de novo and thermostable α-helical barrel, which comprises seven helices arranged around an accessible channel. We show that the lumen of the barrel accepts 21 mutations to functional polar residues. The resulting variant, which has cysteine-histidine-glutamic acid triads on each helix, hydrolyses p-nitrophenyl acetate with catalytic efficiencies that match the most-efficient redesigned hydrolases based on natural protein scaffolds. This is the first report of a functional catalytic triad engineered into a de novo protein framework. The flexibility of our system also allows the facile incorporation of unnatural side chains to improve activity and probe the catalytic mechanism. Such a predictable and robust construction of truly de novo biocatalysts holds promise for applications in chemical and biochemical synthesis.

PubMed: 27554410
DOI: 10.1038/nchem.2555

Experimental method

X-RAY DIFFRACTION (1.8 Å)