5EZC
A de novo designed heptameric coiled coil CC-Hept-I18C-L22H-I25E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2014-05-14 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 62.252, 60.880, 69.380 |
| Unit cell angles | 90.00, 110.89, 90.00 |
Refinement procedure
| Resolution | 44.376 - 1.800 |
| R-factor | 0.1888 |
| Rwork | 0.187 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pna |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.819 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.900 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.475 |
| Number of reflections | 22242 | |
| <I/σ(I)> | 7.7 | 2.3 |
| Completeness [%] | 98.9 | 97.8 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.2 M MgCl2, 0.1 M Tris at pH 7.0 with 10% w/v PEG |
