5ERF

Ketosynthase from module 6 of the bacillaene synthase from Bacillus subtilis 168

Summary for 5ERF

• Entry DOI: 10.2210/pdb5erf/pdb
• Related: 5ELP 5ENY 5ERB
• Descriptor: Polyketide synthase PksL (1 entity in total)
• Functional Keywords: trans-at, ketosynthase, polyketide, bacillaene, hydrolase
• Biological source: Bacillus subtilis (strain 168)
• Total number of polymer chains: 2
• Total formula weight: 138088.08

Authors

Wagner, D.T.,Gay, D.C.,Keatinge-Clay, A.T. (deposition date: 2015-11-14, release date: 2015-12-23, Last modification date: 2024-10-23)

Primary citation

Gay, D.C.,Wagner, D.T.,Meinke, J.L.,Zogzas, C.E.,Gay, G.R.,Keatinge-Clay, A.T.
The LINKS motif zippers trans-acyltransferase polyketide synthase assembly lines into a biosynthetic megacomplex.
J.Struct.Biol., 193:196-205, 2016

PubMed Abstract: Polyketides such as the clinically-valuable antibacterial agent mupirocin are constructed by architecturally-sophisticated assembly lines known as trans-acyltransferase polyketide synthases. Organelle-sized megacomplexes composed of several copies of trans-acyltransferase polyketide synthase assembly lines have been observed by others through transmission electron microscopy to be located at the Bacillus subtilis plasma membrane, where the synthesis and export of the antibacterial polyketide bacillaene takes place. In this work we analyze ten crystal structures of trans-acyltransferase polyketide synthases ketosynthase domains, seven of which are reported here for the first time, to characterize a motif capable of zippering assembly lines into a megacomplex. While each of the three-helix LINKS (Laterally-INteracting Ketosynthase Sequence) motifs is observed to similarly dock with a spatially-reversed copy of itself through hydrophobic and ionic interactions, the amino acid sequences of this motif are not conserved. Such a code is appropriate for mediating homotypic contacts between assembly lines to ensure the ordered self-assembly of a noncovalent, yet tightly-knit, enzymatic network. LINKS-mediated lateral interactions would also have the effect of bolstering the vertical association of the polypeptides that comprise a polyketide synthase assembly line.

PubMed: 26724270
DOI: 10.1016/j.jsb.2015.12.011

Experimental method

X-RAY DIFFRACTION (3.1 Å)