5ERF
Ketosynthase from module 6 of the bacillaene synthase from Bacillus subtilis 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.057, 77.317, 85.291 |
| Unit cell angles | 77.91, 70.26, 64.18 |
Refinement procedure
| Resolution | 39.730 - 3.100 |
| R-factor | 0.22121 |
| Rwork | 0.219 |
| R-free | 0.26841 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.441 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.730 |
| High resolution limit [Å] | 3.100 |
| Number of reflections | 24128 |
| <I/σ(I)> | 2.81 |
| Completeness [%] | 94.1 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 150 mM (NH4)2SO4, 15% PEG 4000 (v/v), 100 mM MES pH 6.0 mixed with 15 mg/ml protein in 150 NaCl and 20 mM HEPES pH 7.5 in 1:2 ratio |
