5EPI

CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT

This is a large structure.

Summary for 5EPI

DescriptorPolymerase acidic protein, RNA-directed RNA polymerase catalytic subunit, Polymerase basic protein 2, ... (4 entities in total)
Functional Keywordsinfluenza b virus, rna-dependent rna polymerase, heterotrimer, subunits pa, pb1, pb2, viral rna, crna 5' end, influenza, transferase
Biological sourceInfluenza B virus (B/Memphis/13/2003)
Total number of polymer chains24
Total molecular weight1600856.36
Authors
Guilligay, D.,Cusack, S. (deposition date: 2015-11-11, release date: 2016-01-13, Last modification date: 2019-04-03)
Primary citation
Thierry, E.,Guilligay, D.,Kosinski, J.,Bock, T.,Gaudon, S.,Round, A.,Pflug, A.,Hengrung, N.,El Omari, K.,Baudin, F.,Hart, D.J.,Beck, M.,Cusack, S.
Influenza Polymerase Can Adopt an Alternative Configuration Involving a Radical Repacking of PB2 Domains.
Mol.Cell, 61:125-137, 2016
PubMed: 26711008 (PDB entries with the same primary citation)
DOI: 10.1016/j.molcel.2015.11.016
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (4.1 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliersRNA backbone 0.2831 1.3% 2.2% 1.4% 0.19MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution