5EPI
CRYSTAL STRUCTURE OF INFLUENZA B POLYMERASE WITH BOUND 5' CRNA EXHIBITS A NOVEL DOMAIN ARRANGEMENT
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07228 |
| Spacegroup name | P 1 |
| Unit cell lengths | 193.730, 209.980, 210.600 |
| Unit cell angles | 117.71, 92.81, 113.68 |
Refinement procedure
| Resolution | 49.945 - 4.100 |
| R-factor | 0.2584 |
| Rwork | 0.257 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wsa |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.539 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.300 |
| High resolution limit [Å] | 4.100 | 4.100 |
| Rmerge | 0.090 | 0.630 |
| Number of reflections | 195792 | |
| <I/σ(I)> | 5.49 | 1.23 |
| Completeness [%] | 92.9 | 94.9 |
| Redundancy | 1.75 | 1.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 100 MM TRI SODIUM CITRATE PH 5.6, 10 OR 100 MM NACL AND 12% MPD |
