5EJM

ThDP-Mn2+ complex of R413A variant of EcMenD soaked with 2-ketoglutarate for 35 min

Summary for 5EJM

• Entry DOI: 10.2210/pdb5ejm/pdb
• Related: 5EJ4 5EJ5 5EJ6 5EJ7 5EJ8 5EJ9 5EJA
• Descriptor: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase, (4~{R})-4-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-5-[2-[oxidanyl(phosphonooxy)phosphoryl]oxyethyl]-1,3-thiazol-3-ium-2-yl]-4-oxidanyl-butanoic acid, MANGANESE (II) ION, ... (6 entities in total)
• Functional Keywords: post-decarboxylation intermediate of r413a variant of ecmend, transferase
• Biological source: Escherichia coli K12
• Total number of polymer chains: 8
• Total formula weight: 496673.34

Authors

Song, H.G.,Dong, C.,Chen, Y.Z.,Sun, Y.R.,Guo, Z.H. (deposition date: 2015-11-02, release date: 2016-11-02, Last modification date: 2024-03-20)

• Primary citation: Qin, M.M.,Song, H.G.,Dai, X.,Chen, Y.Z.,Guo, Z.H.; Two active site arginines are critical determinants of substrate binding and catalysis in MenD, a thiamine-dependent enzyme in menaquinone biosynthesis.; Biochem. J., 2018; PubMed: 30341164; DOI: 10.1042/BCJ20180548

Experimental method

X-RAY DIFFRACTION (1.72 Å)