5EJM
ThDP-Mn2+ complex of R413A variant of EcMenD soaked with 2-ketoglutarate for 35 min
Summary for 5EJM
Entry DOI | 10.2210/pdb5ejm/pdb |
Related | 5EJ4 5EJ5 5EJ6 5EJ7 5EJ8 5EJ9 5EJA |
Descriptor | 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase, (4~{R})-4-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-5-[2-[oxidanyl(phosphonooxy)phosphoryl]oxyethyl]-1,3-thiazol-3-ium-2-yl]-4-oxidanyl-butanoic acid, MANGANESE (II) ION, ... (6 entities in total) |
Functional Keywords | post-decarboxylation intermediate of r413a variant of ecmend, transferase |
Biological source | Escherichia coli K12 |
Total number of polymer chains | 8 |
Total formula weight | 496673.34 |
Authors | Song, H.G.,Dong, C.,Chen, Y.Z.,Sun, Y.R.,Guo, Z.H. (deposition date: 2015-11-02, release date: 2016-11-02, Last modification date: 2024-03-20) |
Primary citation | Qin, M.M.,Song, H.G.,Dai, X.,Chen, Y.Z.,Guo, Z.H. Two active site arginines are critical determinants of substrate binding and catalysis in MenD, a thiamine-dependent enzyme in menaquinone biosynthesis. Biochem. J., 2018 Cited by PubMed: 30341164DOI: 10.1042/BCJ20180548 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.72 Å) |
Structure validation
Download full validation report