5E8H

Crystal structure of geranylfarnesyl pyrophosphate synthases 2 from Arabidopsis thaliana

Summary for 5E8H

• Entry DOI: 10.2210/pdb5e8h/pdb
• Related: 5E8K 5E8L
• Descriptor: Geranylgeranyl pyrophosphate synthase 3, chloroplastic (2 entities in total)
• Functional Keywords: gfpps, prenyltransferase, transferase
• Biological source: Arabidopsis thaliana (Mouse-ear cress)
• Total number of polymer chains: 2
• Total formula weight: 71656.84

Authors

Wang, C.,Chen, Q.,Wang, G.,Zhang, P. (deposition date: 2015-10-14, release date: 2015-11-11, Last modification date: 2024-11-20)

Primary citation

Wang, C.,Chen, Q.,Fan, D.,Li, J.,Wang, G.,Zhang, P.
Structural Analyses of Short-Chain Prenyltransferases Identify an Evolutionarily Conserved GFPPS Clade in Brassicaceae Plants.
Mol Plant, 9:195-204, 2016

PubMed Abstract: Terpenoids are the largest and most diverse class of plant-specialized metabolites, which function in diverse physiological processes during plant development. In the biosynthesis of plant terpenoids, short-chain prenyltransferases (SC-PTs), together with terpene synthases (TPSs), play critical roles in determining terpenoid diversity. SC-PTs biosynthesize prenyl pyrophosphates with different chain lengths, and these compounds are the direct precursors of terpenoids. Arabidopsis thaliana possesses a subgroup of SC-PTs whose functions are not clearly known. In this study, we focus on 10 geranylgeranyl pyrophosphate synthase-like [GGPPSL] proteins, which are commonly thought to produce GGPP [C20]. We found that a subset of members of the Arabidopsis GGPPSL gene family have undergone neo-functionalization: GGPPSL6, 7, 9, and 10 mainly have geranylfarnesyl pyrophosphate synthase activity (C25; renamed AtGFPPS1, 2, 3, and 4), and GGPPSL8 produces even longer chain prenyl pyrophosphate (≥ C30; renamed polyprenyl pyrophosphate synthase 2, AtPPPS2). By solving the crystal structures of AtGFPPS2, AtPPPS2, and AtGGPPS11, we reveal the product chain-length determination mechanism of SC-PTs and interpret it as a "three floors" model. Using this model, we identified a novel GFPPS clade distributed in Brassicaceae plants and found that the GFPPS gene typically occurs in tandem with a gene encoding a TPS, forming a GFPPS-TPS gene cluster.

PubMed: 26537048
DOI: 10.1016/j.molp.2015.10.010

Experimental method

X-RAY DIFFRACTION (2.3 Å)