5E8H

Crystal structure of geranylfarnesyl pyrophosphate synthases 2 from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	80
Detector technology	CCD
Collection date	2013-10-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	77.245, 77.090, 114.172
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.520 - 2.300
R-factor	0.2082
Rwork	0.207
R-free	0.23370
RMSD bond length	0.010
RMSD bond angle	1.473
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	28044
<I/σ(I)>	28.3
Completeness [%]	90.1
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	2.0 M ammonium sulfate, 2% PEG 400, pH7.5