5E5A

Crystal structure of the chromatin-tethering domain of Human cytomegalovirus IE1 protein bound to the nucleosome core particle

5E5A の概要

• エントリーDOI: 10.2210/pdb5e5a/pdb
• 分子名称: DNA (146-MER), Histone H3.2, Histone H4, ... (8 entities in total)
• 機能のキーワード: ncp, ie1, hcmv, ctd, protein binding-viral protein-dna complex, protein binding/viral protein/dna
• 由来する生物種: Homo sapiens (Humam); 詳細
• 細胞内の位置: Nucleus: P84233 P62799 P02281
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 201100.70

構造登録者

Fang, Q.,Chen, P.,Wang, M.,Fang, J.,Yang, N.,Li, G.,Xu, R.M. (登録日: 2015-10-08, 公開日: 2016-02-03, 最終更新日: 2023-11-08)

主引用文献

Fang, Q.,Chen, P.,Wang, M.,Fang, J.,Yang, N.,Li, G.,Xu, R.M.
Human cytomegalovirus IE1 protein alters the higher-order chromatin structure by targeting the acidic patch of the nucleosome
Elife, 5:-, 2016

PubMed Abstract: Human cytomegalovirus (hCMV) immediate early 1 (IE1) protein associates with condensed chromatin of the host cell during mitosis. We have determined the structure of the chromatin-tethering domain (CTD) of IE1 bound to the nucleosome core particle, and discovered that IE1-CTD specifically interacts with the H2A-H2B acidic patch and impairs the compaction of higher-order chromatin structure. Our results suggest that IE1 loosens up the folding of host chromatin during hCMV infections.

PubMed: 26812545
DOI: 10.7554/eLife.11911

実験手法

X-RAY DIFFRACTION (2.809 Å)