5DV7

Crystal Structure of NF90 tandem dsRBDs with dsRNA

Summary for 5DV7

• Entry DOI: 10.2210/pdb5dv7/pdb
• Descriptor: RNA (5'-R(*CP*CP*AP*GP*CP*AP*UP*UP*AP*UP*GP*AP*AP*AP*GP*UP*GP*A)-3'), RNA (5'-R(*UP*CP*AP*CP*UP*UP*UP*CP*AP*UP*AP*AP*UP*GP*CP*UP*GP*G)-3'), Interleukin enhancer-binding factor 3, ... (4 entities in total)
• Functional Keywords: double stranded rna binding domains, dsrm, dsrbds, dsrna, nf90, ilf3, drbp76, rna binding protein
• Biological source: Mus musculus (Mouse); More
• Cellular location: Nucleus, nucleolus : Q9Z1X4
• Total number of polymer chains: 3
• Total formula weight: 87524.56

Authors

Jayachandran, U.,Grey, H.,Cook, A.G. (deposition date: 2015-09-21, release date: 2016-01-13, Last modification date: 2024-01-10)

Primary citation

Jayachandran, U.,Grey, H.,Cook, A.G.
Nuclear factor 90 uses an ADAR2-like binding mode to recognize specific bases in dsRNA.
Nucleic Acids Res., 44:1924-1936, 2016

PubMed Abstract: Nuclear factors 90 and 45 (NF90 and NF45) form a protein complex involved in the post-transcriptional control of many genes in vertebrates. NF90 is a member of the dsRNA binding domain (dsRBD) family of proteins. RNA binding partners identified so far include elements in 3' untranslated regions of specific mRNAs and several non-coding RNAs. In NF90, a tandem pair of dsRBDs separated by a natively unstructured segment confers dsRNA binding activity. We determined a crystal structure of the tandem dsRBDs of NF90 in complex with a synthetic dsRNA. This complex shows surprising similarity to the tandem dsRBDs from an adenosine-to-inosine editing enzyme, ADAR2 in complex with a substrate RNA. Residues involved in unusual base-specific recognition in the minor groove of dsRNA are conserved between NF90 and ADAR2. These data suggest that, like ADAR2, underlying sequences in dsRNA may influence how NF90 recognizes its target RNAs.

PubMed: 26712564
DOI: 10.1093/nar/gkv1508

Experimental method

X-RAY DIFFRACTION (3.5 Å)