5DV7
Crystal Structure of NF90 tandem dsRBDs with dsRNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.17 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 100.104, 100.104, 108.007 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.086 | 0.912 |
Number of reflections | 7981 | |
<I/σ(I)> | 22.1 | 3.6 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 50mM sodium citrate pH 5.0, 1.0M ammonium formate |