5DLG

Crystal Structure of Human DNA Polymerase Eta Inserting dGMPNPP Opposite O4-Methylhymidine

Summary for 5DLG

Related5DLF
DescriptorDNA polymerase eta, DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*T)-3'), DNA (5'-D(*CP*AP*TP*(5DB)P*AP*TP*GP*AP*CP*GP*CP*T)-3'), ... (7 entities in total)
Functional Keywordscatalytic domain, dna damage, dna-directed dna polymerase, guanosine triphosphate, y-family polymerase, trans-lesion synthesis (tls), dna binding, o4-alkylthymidine, o4-methylthymidine, transferase-dna complex, transferase/dna
Biological sourceHomo sapiens (Human)
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Total number of polymer chains3
Total molecular weight55342.64
Authors
Patra, A.,OFlaherty, D.K.,Egli, M. (deposition date: 2015-09-04, release date: 2016-08-10, Last modification date: 2019-01-16)
Primary citation
O'Flaherty, D.K.,Patra, A.,Su, Y.,Guengerich, F.P.,Egli, M.,Wilds, C.J.
Lesion Orientation ofO4-Alkylthymidine Influences Replication by Human DNA Polymeraseeta.
Chem Sci, 7:4896-4904, 2016
PubMed: 27574558 (PDB entries with the same primary citation)
DOI: 10.1039/C6SC00666C
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (2.351 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2496 0.7% 2.4% 5.6%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution