5DGK
SCCmec type IV Cch - active helicase
Summary for 5DGK
| Entry DOI | 10.2210/pdb5dgk/pdb |
| Descriptor | Active helicase, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (4 entities in total) |
| Functional Keywords | active ring shaped helicase, replication |
| Biological source | Staphylococcus aureus |
| Total number of polymer chains | 2 |
| Total formula weight | 137690.85 |
| Authors | Rice, P.A.,Mir-Sanchis, I. (deposition date: 2015-08-27, release date: 2016-08-24, Last modification date: 2024-10-30) |
| Primary citation | Mir-Sanchis, I.,Roman, C.A.,Misiura, A.,Pigli, Y.Z.,Boyle-Vavra, S.,Rice, P.A. Staphylococcal SCCmec elements encode an active MCM-like helicase and thus may be replicative. Nat.Struct.Mol.Biol., 23:891-898, 2016 Cited by PubMed Abstract: Methicillin-resistant Staphylococcus aureus (MRSA) is a public-health threat worldwide. Although the mobile genomic island responsible for this phenotype, staphylococcal cassette chromosome (SCC), has been thought to be nonreplicative, we predicted DNA-replication-related functions for some of the conserved proteins encoded by SCC. We show that one of these, Cch, is homologous to the self-loading initiator helicases of an unrelated family of genomic islands, that it is an active 3'-to-5' helicase and that the adjacent ORF encodes a single-stranded DNA-binding protein. Our 2.9-Å crystal structure of intact Cch shows that it forms a hexameric ring. Cch, like the archaeal and eukaryotic MCM-family replicative helicases, belongs to the pre-sensor II insert clade of AAA+ ATPases. Additionally, we found that SCC elements are part of a broader family of mobile elements, all of which encode a replication initiator upstream of their recombinases. Replication after excision would enhance the efficiency of horizontal gene transfer. PubMed: 27571176DOI: 10.1038/nsmb.3286 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.895 Å) |
Structure validation
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