5DGK
SCCmec type IV Cch - active helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-07-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | H 3 |
Unit cell lengths | 110.140, 110.140, 302.755 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.177 - 2.895 |
R-factor | 0.224 |
Rwork | 0.221 |
R-free | 0.26110 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.963 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_1839) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.177 | 2.950 |
High resolution limit [Å] | 2.890 | 2.900 |
Number of reflections | 28123 | |
<I/σ(I)> | 19 | |
Completeness [%] | 92.2 | 64.16 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 292 | 32% PEG 200, 0.1M HEPES, pH 7.4 |