5DBX

Crystal structure of murine SPAK(T243D) in complex with AMPPNP

5DBX の概要

• エントリーDOI: 10.2210/pdb5dbx/pdb
• 分子名称: STE20/SPS1-related proline-alanine-rich protein kinase, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: kinase, transferase
• 由来する生物種: Mus musculus (Mouse)
• 細胞内の位置: Cytoplasm : Q9Z1W9
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 75570.66

構造登録者

Juang, Y.-C. (登録日: 2015-08-22, 公開日: 2015-09-23, 最終更新日: 2024-11-20)

主引用文献

Taylor, C.A.,Juang, Y.C.,Earnest, S.,Sengupta, S.,Goldsmith, E.J.,Cobb, M.H.
Domain-Swapping Switch Point in Ste20 Protein Kinase SPAK.
Biochemistry, 54:5063-5071, 2015

PubMed Abstract: The related protein kinases SPAK and OSR1 regulate ion homeostasis in part by phosphorylating cation cotransporter family members. The structure of the kinase domain of OSR1 was determined in the unphosphorylated inactive form and, like some other Ste20 kinases, exhibited a domain-swapped activation loop. To further probe the role of domain swapping in SPAK and OSR1, we have determined the crystal structures of SPAK 63-403 at 3.1 Å and SPAK 63-390 T243D at 2.5 Å resolution. These structures encompass the kinase domain and different portions of the C-terminal tail, the longer without and the shorter with an activating T243D point mutation. The structure of the T243D protein reveals significant conformational differences relative to unphosphorylated SPAK and OSR1 but also has some features of an inactive kinase. Both structures are domain-swapped dimers. Sequences involved in domain swapping were identified and mutated to create a SPAK monomeric mutant with kinase activity, indicating that monomeric forms are active. The monomeric mutant is activated by WNK1 but has reduced activity toward its substrate NKCC2, suggesting regulatory roles for domain swapping. The structure of partially active SPAK T243D is consistent with a multistage activation process in which phosphorylation induces a SPAK conformation that requires further remodeling to build the active structure.

PubMed: 26208601
DOI: 10.1021/acs.biochem.5b00593

実験手法

X-RAY DIFFRACTION (2.5 Å)