5D7V

Crystal structure of PTK6 kinase domain

5D7V の概要

• エントリーDOI: 10.2210/pdb5d7v/pdb
• 分子名称: Protein-tyrosine kinase 6, PHOSPHATE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: transferase, kinase, brk, apo
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm: Q13882
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 126048.65

構造登録者

Thakur, M.K.,Birudukota, S.,Swaminathan, S.,Tyagi, R.,Gosu, R. (登録日: 2015-08-14, 公開日: 2016-08-17, 最終更新日: 2024-11-13)

主引用文献

Thakur, M.K.,Kumar, A.,Birudukota, S.,Swaminathan, S.,Tyagi, R.,Gosu, R.
Crystal structure of the kinase domain of human protein tyrosine kinase 6 (PTK6) at 2.33 angstrom resolution
Biochem.Biophys.Res.Commun., 478:637-642, 2016

PubMed Abstract: Human Protein tyrosine kinase 6 (PTK6) (EC:2.7.10.2), also known as the breast tumor kinase (BRK), is an intracellular non-receptor Src-related tyrosine kinase expressed in a majority of human breast tumors and breast cancer cell lines, but its expression is low or completely absent in normal mammary glands. In the recent past, several studies have suggested that PTK6 is a potential therapeutic target in cancer. To understand its structural and functional properties, the PTK6 kinase domain (PTK6-KD) gene was cloned, overexpressed in a baculo-insect cell system, purified and crystallized at room temperature. X-ray diffraction data to 2.33 Å resolution was collected on a single PTK6-KD crystal, which belonged to the triclinic space group P1. The Matthews coefficient calculation suggested the presence of four protein molecules per asymmetric unit, with a solvent content of ∼50%.The structure has been solved by molecular replacement and crystal structure data submitted to the protein data bank under the accession number 5D7V. This is the first report of apo PTK6-KD structure crystallized in DFG-in and αC-helix-out conformation.

PubMed: 27480927
DOI: 10.1016/j.bbrc.2016.07.121

実験手法

X-RAY DIFFRACTION (2.33 Å)