5CMQ
Crystal Structure of Zn-bound Human H-Ferritin variant 122H-delta C-star
Summary for 5CMQ
| Entry DOI | 10.2210/pdb5cmq/pdb |
| Related | 5CMR |
| Descriptor | Ferritin heavy chain, ZINC ION (3 entities in total) |
| Functional Keywords | protein engineering, metal binding, oxidoreductase |
| Biological source | Homo sapiens (Human) |
| Total number of polymer chains | 1 |
| Total formula weight | 21710.97 |
| Authors | Sontz, P.A.,Bailey, J.B.,Ahn, S.,Tezcan, F.A. (deposition date: 2015-07-17, release date: 2015-09-09, Last modification date: 2023-09-27) |
| Primary citation | Sontz, P.A.,Bailey, J.B.,Ahn, S.,Tezcan, F.A. A Metal Organic Framework with Spherical Protein Nodes: Rational Chemical Design of 3D Protein Crystals. J.Am.Chem.Soc., 137:11598-11601, 2015 Cited by PubMed Abstract: We describe here the construction of a three-dimensional, porous, crystalline framework formed by spherical protein nodes that assemble into a prescribed lattice arrangement through metal-organic linker-directed interactions. The octahedral iron storage enzyme, ferritin, was engineered in its C3 symmetric pores with tripodal Zn coordination sites. Dynamic light scattering and crystallographic studies established that this Zn-ferritin construct could robustly self-assemble into the desired bcc-type crystals upon coordination of a ditopic linker bearing hydroxamic acid functional groups. This system represents the first example of a ternary protein-metal-organic crystalline framework whose formation is fully dependent on each of its three components. PubMed: 26305584DOI: 10.1021/jacs.5b07463 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.935 Å) |
Structure validation
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