5C5Y

Crystal structure of deoxyribose-phosphate aldolase from Colwellia psychrerythraea (hexagonal form)

Summary for 5C5Y

• Entry DOI: 10.2210/pdb5c5y/pdb
• Related: 5C2X
• Descriptor: Deoxyribose-phosphate aldolase, UNKNOWN LIGAND, GLYCEROL, ... (4 entities in total)
• Functional Keywords: dera, tim barrel, psychrophilic, lyase
• Biological source: Colwellia psychrerythraea (strain 34H / ATCC BAA-681)
• Total number of polymer chains: 4
• Total formula weight: 113689.46

Authors

Weiergraeber, O.H.,Dick, M.,Pietruszka, J. (deposition date: 2015-06-22, release date: 2016-02-03, Last modification date: 2024-01-10)

Primary citation

Dick, M.,Weiergraber, O.H.,Classen, T.,Bisterfeld, C.,Bramski, J.,Gohlke, H.,Pietruszka, J.
Trading off stability against activity in extremophilic aldolases.
Sci Rep, 6:17908-17908, 2016

PubMed Abstract: Understanding enzyme stability and activity in extremophilic organisms is of great biotechnological interest, but many questions are still unsolved. Using 2-deoxy-D-ribose-5-phosphate aldolase (DERA) as model enzyme, we have evaluated structural and functional characteristics of different orthologs from psychrophilic, mesophilic and hyperthermophilic organisms. We present the first crystal structures of psychrophilic DERAs, revealing a dimeric organization resembling their mesophilic but not their thermophilic counterparts. Conversion into monomeric proteins showed that the native dimer interface contributes to stability only in the hyperthermophilic enzymes. Nevertheless, introduction of a disulfide bridge in the interface of a psychrophilic DERA did confer increased thermostability, suggesting a strategy for rational design of more durable enzyme variants. Constraint network analysis revealed particularly sparse interactions between the substrate pocket and its surrounding α-helices in psychrophilic DERAs, which indicates that a more flexible active center underlies their high turnover numbers.

PubMed: 26783049
DOI: 10.1038/srep17908

Experimental method

X-RAY DIFFRACTION (2.1 Å)