5BKA

2.1 Angstrom structure of ActVI-ORFA from Streptomyces Coelicolor

Summary for 5BKA

• Entry DOI: 10.2210/pdb5bka/pdb
• Descriptor: Hydroxylacyl-CoA dehydrogenase (2 entities in total)
• Functional Keywords: actinorhodin, benzoisochromaquinone, oxidoreductase
• Biological source: Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
• Total number of polymer chains: 6
• Total formula weight: 97752.10

Authors

Vuksanovic, N.,Silvaggi, N.R. (deposition date: 2019-06-02, release date: 2020-06-03, Last modification date: 2024-10-30)

Primary citation

Vuksanovic, N.,Zhu, X.,Serrano, D.A.,Siitonen, V.,Metsa-Ketela, M.,Melancon 3rd, C.E.,Silvaggi, N.R.
Structural characterization of three noncanonical NTF2-like superfamily proteins: implications for polyketide biosynthesis.
Acta Crystallogr.,Sect.F, 76:372-383, 2020

PubMed Abstract: Proteins belonging to the NTF2-like superfamily are present in the biosynthetic pathways of numerous polyketide natural products, such as anthracyclins and benzoisochromanequinones. Some have been found to be bona fide polyketide cyclases, but many of them have roles that are currently unknown. Here, the X-ray crystal structures of three NTF2-like proteins of unknown function are reported: those of ActVI-ORFA from Streptomyces coelicolor A3(2) and its homologs Caci_6494, a protein from an uncharacterized biosynthetic cluster in Catenulispora acidiphila, and Aln2 from Streptomyces sp. CM020, a protein in the biosynthetic pathway of alnumycin. The presence of a solvent-accessible cavity and the conservation of the His/Asp dyad that is characteristic of many polyketide cyclases suggest a potential enzymatic role for these enzymes in polyketide biosynthesis.

PubMed: 32744249
DOI: 10.1107/S2053230X20009814

Experimental method

X-RAY DIFFRACTION (2.11 Å)