5BKA
2.1 Angstrom structure of ActVI-ORFA from Streptomyces Coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 82.266, 52.916, 98.931 |
| Unit cell angles | 90.00, 104.33, 90.00 |
Refinement procedure
| Resolution | 34.910 - 2.110 |
| R-factor | 0.2254 |
| Rwork | 0.224 |
| R-free | 0.24670 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.812 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.940 | 2.185 |
| High resolution limit [Å] | 2.110 | 2.110 |
| Rmeas | 0.096 | 0.840 |
| Number of reflections | 47829 | 3240 |
| <I/σ(I)> | 8.31 | |
| Completeness [%] | 95.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 20% PEG 3350, 0.1 M Sodium Citrate pH 4.5 |
