5B7I

Cas3-AcrF3 complex

Summary for 5B7I

• Entry DOI: 10.2210/pdb5b7i/pdb
• Descriptor: CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST, Uncharacterized protein AcrF3, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: dna nuclease, phagy protein, anti-crispr, hydrolase-unknown function complex, hydrolase/unknown function
• Biological source: Pseudomonas aeruginosa (strain UCBPP-PA14); More
• Total number of polymer chains: 3
• Total formula weight: 158287.49

Authors

Wang, X.,Zhu, Y. (deposition date: 2016-06-07, release date: 2016-07-06, Last modification date: 2024-10-23)

Primary citation

Wang, X.,Yao, D.,Xu, J.G.,Li, A.R.,Xu, J.,Fu, P.,Zhou, Y.,Zhu, Y.
Structural basis of Cas3 inhibition by the bacteriophage protein AcrF3
Nat.Struct.Mol.Biol., 23:868-870, 2016

PubMed Abstract: Bacteriophages express proteins that inactivate the CRISPR-Cas bacterial immune system. Here we report the crystal structure of the anti-CRISPR protein AcrF3 in complex with Pseudomonas aeruginosa Cas3 (PaCas3). AcrF3 forms a homodimer that locks PaCas3 in an ADP-bound form, blocks the entrance of the DNA-binding tunnel in the helicase domain, and masks the linker region and C-terminal domain of PaCas3, thereby preventing recruitment by Cascade and inhibiting the type I-F CRISPR-Cas system.

PubMed: 27455460
DOI: 10.1038/nsmb.3269

Experimental method

X-RAY DIFFRACTION (2.6 Å)