5B7I

Cas3-AcrF3 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	CRISPR-associated nuclease/helicase Cas3 subtype I-F/YPEST	polymer	1082	122554.6	1	UniProt (Q02ML8) Pfam (PF21384) Pfam (PF18019) Pfam (PF22590) Pfam (PF21802) In PDB	Pseudomonas aeruginosa (strain UCBPP-PA14)
2	B, C	Uncharacterized protein AcrF3	polymer	153	17612.8	2	UniProt (L7P7R7) Pfam (PF21401) In PDB	Pseudomonas phage JBD5
3	A	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
4	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
5		water	water		18.0	85	Chemie (HOH)

Sequence modifications

A: 1 - 1076 (UniProt: Q02ML8)

PDB	External Database	Details
Pro -5	-	expression tag
Leu -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Gly 529	Arg 529	see sequence details

B, C: 1 - 139 (UniProt: L7P7R7)

PDB	External Database	Details
Mse -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	157780.1
Non-Polymers*	Number of molecules	3
	Total formula weight	507.4
All*	Total formula weight	158287.5

*Water molecules are not included.