5B5G

Crystal structure of ALiS4-Streptavidin complex

Summary for 5B5G

Related5B5F
DescriptorStreptavidin, SULFITE ION, methyl 5-(4-oxidanylidene-5~{H}-furo[3,2-c]pyridin-2-yl)pyridine-3-carboxylate, ... (4 entities in total)
Functional Keywordsbiotin-binding protein, high-strength agarose hydrogel
Biological sourceStreptomyces avidinii
Cellular locationSecreted P22629
Total number of polymer chains4
Total molecular weight51875.95
Authors
Sugiyama, S.,Terai, T.,Kakinouchi, K.,Fujikake, R.,Nagano, T.,Urano, Y. (deposition date: 2016-05-04, release date: 2017-03-01)
Primary citation
Tachibana, R.,Terai, T.,Boncompain, G.,Sugiyama, S.,Saito, N.,Perez, F.,Urano, Y.
Improving the Solubility of Artificial Ligands of Streptavidin to Enable More Practical Reversible Switching of Protein Localization in Cells
Chembiochem, 18:358-362, 2017
PubMed: 27905160 (PDB entries with the same primary citation)
DOI: 10.1002/cbic.201600640
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.5 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.19480.2%2.0%3.5%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5b5g
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Molmil generated image of 5b5g
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Molmil generated image of 5b5g
rotated about y axis by 90°