5B5F

Crystal structure of ALiS3-Streptavidin complex

Summary for 5B5F

• Entry DOI: 10.2210/pdb5b5f/pdb
• Related: 4Y59 4Y5D 5B5G
• Descriptor: Streptavidin, N-methyl-3-(4-oxo-4,5-dihydrofuro[3,2-c]pyridin-2-yl)benzenesulfonamide (3 entities in total)
• Functional Keywords: biotin-binding protein, high-strength agarose hydrogel
• Biological source: Streptomyces avidinii
• Total number of polymer chains: 4
• Total formula weight: 51992.31

Authors

Sugiyama, S.,Terai, T.,Kakinouchi, K.,Fujikake, R.,Nagano, T.,Urano, Y. (deposition date: 2016-05-04, release date: 2017-03-01, Last modification date: 2023-11-08)

Primary citation

Tachibana, R.,Terai, T.,Boncompain, G.,Sugiyama, S.,Saito, N.,Perez, F.,Urano, Y.
Improving the Solubility of Artificial Ligands of Streptavidin to Enable More Practical Reversible Switching of Protein Localization in Cells
Chembiochem, 18:358-362, 2017

PubMed Abstract: Chemical inducers that can control target-protein localization in living cells are powerful tools to investigate dynamic biological systems. We recently reported the retention using selective hook or "RUSH" system for reversible localization change of proteins of interest by addition/washout of small-molecule artificial ligands of streptavidin (ALiS). However, the utility of previously developed ALiS was restricted by limited solubility in water. Here, we overcame this problem by X-ray crystal structure-guided design of a more soluble ALiS derivative (ALiS-3), which retains sufficient streptavidin-binding affinity for use in the RUSH system. The ALiS-3-streptavidin interaction was characterized in detail. ALiS-3 is a convenient and effective tool for dynamic control of α-mannosidase II localization between ER and Golgi in living cells.

PubMed: 27905160
DOI: 10.1002/cbic.201600640

Experimental method

X-RAY DIFFRACTION (1.2 Å)