5B2P

Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGA PAM)

Summary for 5B2P

Entry DOI	10.2210/pdb5b2p/pdb
Related	5B2O 5B2Q
Descriptor	CRISPR-associated endonuclease Cas9, ACETATE ION, Guide RNA, ... (11 entities in total)
Functional Keywords	crispr-cas9, genome engineering, hydrolase-rna-dna complex, hydrolase/rna/dna
Biological source	Francisella tularensis subsp. novicida U112 More
Total number of polymer chains	4
Total formula weight	235532.22
Authors	Hirano, H.,Nishimasu, H.,Nakane, T.,Ishitani, R.,Nureki, O. (deposition date: 2016-02-01, release date: 2016-03-02, Last modification date: 2024-03-20)
Primary citation	Hirano, H.,Gootenberg, J.S.,Horii, T.,Abudayyeh, O.O.,Kimura, M.,Hsu, P.D.,Nakane, T.,Ishitani, R.,Hatada, I.,Zhang, F.,Nishimasu, H.,Nureki, O. Structure and Engineering of Francisella novicida Cas9 Cell, 164:950-961, 2016 Cited by PubMed: 26875867 DOI: 10.1016/j.cell.2016.01.039 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.7 Å)

Structure validation