5B2O
Crystal structure of Francisella novicida Cas9 in complex with sgRNA and target DNA (TGG PAM)
Summary for 5B2O
Entry DOI | 10.2210/pdb5b2o/pdb |
Related | 5B2P 5B2Q |
Descriptor | CRISPR-associated endonuclease Cas9, ACETATE ION, Guide RNA, ... (11 entities in total) |
Functional Keywords | crispr-cas9, genome engineering, hydrolase-rna-dna complex, hydrolase/rna/dna |
Biological source | Francisella tularensis subsp. novicida U112 More |
Total number of polymer chains | 4 |
Total formula weight | 235510.17 |
Authors | Hirano, H.,Nishimasu, H.,Nakane, T.,Ishitani, R.,Nureki, O. (deposition date: 2016-02-01, release date: 2016-03-02, Last modification date: 2024-03-20) |
Primary citation | Hirano, H.,Gootenberg, J.S.,Horii, T.,Abudayyeh, O.O.,Kimura, M.,Hsu, P.D.,Nakane, T.,Ishitani, R.,Hatada, I.,Zhang, F.,Nishimasu, H.,Nureki, O. Structure and Engineering of Francisella novicida Cas9 Cell, 164:950-961, 2016 Cited by PubMed: 26875867DOI: 10.1016/j.cell.2016.01.039 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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