5AYJ

Hyperthermostable mutant of Bacillus sp. TB-90 Urate Oxidase - R298C

Summary for 5AYJ

• Entry DOI: 10.2210/pdb5ayj/pdb
• Related: 1J2G 3WLV
• Descriptor: Uric acid degradation bifunctional protein, 9-METHYL URIC ACID, HEXAETHYLENE GLYCOL, ... (5 entities in total)
• Functional Keywords: disulfide bond, thermostability, protein engineering, oxidase, subunit-subunit interaction, oxidoreductase
• Biological source: Bacillus sp. TB-90
• Total number of polymer chains: 4
• Total formula weight: 154229.16

Authors

Hibi, T.,Kume, A.,Kawamura, A.,Itoh, T.,Nishiya, Y. (deposition date: 2015-08-21, release date: 2016-01-20, Last modification date: 2023-11-08)

• Primary citation: Hibi, T.,Kume, A.,Kawamura, A.,Itoh, T.,Fukada, H.,Nishiya, Y.; Hyperstabilization of Tetrameric Bacillus sp. TB-90 Urate Oxidase by Introducing Disulfide Bonds through Structural Plasticity; Biochemistry, 55:724-732, 2016; PubMed: 26739254; DOI: 10.1021/acs.biochem.5b01119

Experimental method

X-RAY DIFFRACTION (2.05 Å)