5AYJ

Hyperthermostable mutant of Bacillus sp. TB-90 Urate Oxidase - R298C

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004846	molecular_function	urate oxidase activity
A	0006144	biological_process	purine nucleobase metabolic process
B	0004846	molecular_function	urate oxidase activity
B	0006144	biological_process	purine nucleobase metabolic process
C	0004846	molecular_function	urate oxidase activity
C	0006144	biological_process	purine nucleobase metabolic process
D	0004846	molecular_function	urate oxidase activity
D	0006144	biological_process	purine nucleobase metabolic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	11
Details	binding site for residue MUA A 401

Chain	Residue
A	PHE184
B	ASP74
B	HOH610
A	LEU195
A	ARG201
A	SER248
A	ILE249
A	GLN250
A	ASN276
B	TYR11
B	THR73

---

site_id	AC2
Number of Residues	4
Details	binding site for residue P6G A 402

Chain	Residue
A	LYS50
A	ILE116
B	TYR301
B	PHE303

---

site_id	AC3
Number of Residues	2
Details	binding site for residue SO4 A 403

Chain	Residue
A	GLU231
A	ARG234

---

site_id	AC4
Number of Residues	13
Details	binding site for residue MUA B 401

Chain	Residue
A	TYR11
A	ALA72
A	THR73
A	ASP74
B	PHE184
B	LEU195
B	ARG201
B	SER248
B	ILE249
B	GLN250
B	ASN276
B	HOH585
B	HOH609

---

site_id	AC5
Number of Residues	2
Details	binding site for residue P6G B 402

Chain	Residue
A	TYR301
B	LYS50

---

site_id	AC6
Number of Residues	4
Details	binding site for residue P6G B 403

Chain	Residue
B	GLN168
B	ASN208
B	LYS210
B	HOH560

---

site_id	AC7
Number of Residues	3
Details	binding site for residue P6G B 404

Chain	Residue
B	THR244
B	LEU255
B	ARG259

---

site_id	AC8
Number of Residues	3
Details	binding site for residue SO4 B 405

Chain	Residue
B	PHE37
B	GLU231
B	ARG234

---

site_id	AC9
Number of Residues	12
Details	binding site for residue MUA C 401

Chain	Residue
C	PHE184
C	LEU195
C	ARG201
C	SER248
C	ILE249
C	GLN250
C	ASN276
C	HOH608
D	TYR11
D	ALA72
D	THR73
D	ASP74

---

site_id	AD1
Number of Residues	6
Details	binding site for residue P6G C 402

Chain	Residue
C	TYR151
C	GLN168
C	ASN208
C	LYS210
C	GLU273
C	HOH584

---

site_id	AD2
Number of Residues	2
Details	binding site for residue SO4 C 403

Chain	Residue
C	GLU231
C	ARG234

---

site_id	AD3
Number of Residues	5
Details	binding site for residue P6G D 401

Chain	Residue
C	GLN275
C	TYR301
C	PHE303
D	LYS50
D	HOH502

---

site_id	AD4
Number of Residues	12
Details	binding site for residue MUA D 402

Chain	Residue
C	TYR11
C	THR73
C	ASP74
D	PHE184
D	LEU195
D	ARG201
D	SER248
D	ILE249
D	GLN250
D	ASN276
D	HOH531
D	HOH584

---

site_id	AD5
Number of Residues	2
Details	binding site for residue SO4 D 403

Chain	Residue
D	GLU231
D	ARG234

---

Functional Information from PROSITE/UniProt

site_id	PS00366
Number of Residues	28
Details	URICASE Uricase signature. LqLIKVSgNsFvgFirdeYttLpedsnR

Chain	Residue	Details
A	LEU174-ARG201

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: Charge relay system; for urate oxidase activity => ECO:0000250|UniProtKB:D0VWQ1

Chain	Residue	Details
A	LYS13
A	THR73
B	LYS13
B	THR73
C	LYS13
C	THR73
D	LYS13
D	THR73

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	ACT_SITE: Charge relay system => ECO:0000250|UniProtKB:Q00511

Chain	Residue	Details
A	LYS24
B	LYS24
C	LYS24
D	LYS24

---

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q00511

Chain	Residue	Details
A	THR73
B	ASN276
C	THR73
C	ASP74
C	ILE249
C	GLN250
C	ASN276
D	THR73
D	ASP74
D	ILE249
D	GLN250
A	ASP74
D	ASN276
A	ILE249
A	GLN250
A	ASN276
B	THR73
B	ASP74
B	ILE249
B	GLN250

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305|Ref.3

Chain	Residue	Details
A	PHE184
A	ARG201
B	PHE184
B	ARG201
C	PHE184
C	ARG201
D	PHE184
D	ARG201

---