Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.80 angstrom

Replaces:  3WTA

Summary for 5AX1

Related5AWZ 5AX0
DescriptorRhodopsin I, RETINAL, (2S)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate, ... (8 entities in total)
Functional Keywordsproton transport, membrane protein, retinal, water cluster, green algae, phototaxis, cell-free synthesis, microbial-type rhodopsin, light-driven proton pump
Biological sourceAcetabularia acetabulum (Mermaid's wine glass)
Total number of polymer chains1
Total molecular weight28523.84
Furuse, M.,Hosaka, T.,Kimura-Someya, T.,Yokoyama, S.,Shirouzu, M. (deposition date: 2015-07-10, release date: 2015-08-26, Last modification date: 2015-11-18)
Primary citation
Furuse, M.,Tamogami, J.,Hosaka, T.,Kikukawa, T.,Shinya, N.,Hato, M.,Ohsawa, N.,Kim, S.Y.,Jung, K.H.,Demura, M.,Miyauchi, S.,Kamo, N.,Shimono, K.,Kimura-Someya, T.,Yokoyama, S.,Shirouzu, M.
Structural basis for the slow photocycle and late proton release in Acetabularia rhodopsin I from the marine plant Acetabularia acetabulum
Acta Crystallogr.,Sect.D, 71:2203-2216, 2015
PubMed: 26527138 (PDB entries with the same primary citation)
DOI: 10.1107/S1399004715015722
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.20320.4%01.7%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 5ax1
no rotation
Molmil generated image of 5ax1
rotated about x axis by 90°
Molmil generated image of 5ax1
rotated about y axis by 90°
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