5AX1
Crystal Structure of the Cell-Free Synthesized Membrane Protein, Acetabularia Rhodopsin I, at 1.80 angstrom
Replaces: 3WTAExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-31 |
Detector | RAYONIX MX325HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 75.990, 101.630, 43.760 |
Unit cell angles | 90.00, 119.17, 90.00 |
Refinement procedure
Resolution | 34.826 - 1.803 |
R-factor | 0.1761 |
Rwork | 0.175 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3w |
RMSD bond length | 0.007 |
RMSD bond angle | 1.073 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1690) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.830 | 1.870 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 26610 | |
<I/σ(I)> | 13.55 | 1.91 |
Completeness [%] | 99.5 | 96.16 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 8 | 293 | 20% PEG400, 0.1M Tris, 0.1M NaCl, 20mM MgCl2 |