5AVN

The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel

Summary for 5AVN

• Entry DOI: 10.2210/pdb5avn/pdb
• Related: 5AVD 5AVG 5AVH
• Descriptor: Xylose isomerase, MANGANESE (II) ION, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: xylose isomerase, hydrogel, isomerase
• Biological source: Streptomyces rubiginosus
• Total number of polymer chains: 2
• Total formula weight: 86878.49

Authors

Sugiyama, S.,Shimizu, N.,Maruyama, N.,Sazaki, G.,Adachi, H.,Takano, K.,Murakami, S.,Inoue, T.,Mori, Y.,Matsumura, H. (deposition date: 2015-06-23, release date: 2015-07-08, Last modification date: 2023-11-08)

Primary citation

Sugiyama, S.,Maruyama, M.,Sazaki, G.,Hirose, M.,Adachi, H.,Takano, K.,Murakami, S.,Inoue, T.,Mori, Y.,Matsumura, H.
Growth of protein crystals in hydrogels prevents osmotic shock
J.Am.Chem.Soc., 134:5786-5789, 2012

PubMed Abstract: High-throughput protein X-ray crystallography offers a significant opportunity to facilitate drug discovery. The most reliable approach is to determine the three-dimensional structure of the protein-ligand complex by soaking the ligand in apo crystals. However, protein apo crystals produced by conventional crystallization in a solution are fatally damaged by osmotic shock during soaking. To overcome this difficulty, we present a novel technique for growing protein crystals in a high-concentration hydrogel that is completely gellified and exhibits high strength. This technique allowed us essentially to increase the mechanical stability of the crystals, preventing serious damage to the crystals caused by osmotic shock. Thus, this method may accelerate structure-based drug discoveries.

PubMed: 22435400
DOI: 10.1021/ja301584y

Experimental method

X-RAY DIFFRACTION (1.03 Å)