5AVN
The 1.03 angstrom structure (P212121) of glucose isomerase crystallized in high-strength agarose hydrogel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-09-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.90 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 94.082, 98.285, 85.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.030 |
R-factor | 0.1286 |
Rwork | 0.127 |
R-free | 0.14969 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1o1h |
RMSD bond length | 0.022 |
RMSD bond angle | 2.037 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.070 |
High resolution limit [Å] | 1.030 | 1.030 |
Rmerge | 0.057 | 0.407 |
Number of reflections | 386570 | |
<I/σ(I)> | 9.1 | 1.9 |
Completeness [%] | 99.2 | 99.8 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 293 | 0.1 M Hepes-NaOH (pH 7.5), 1.75 M (NH4)2SO4 |